The actinomycete isolate M20 was isolated by dry heat (70 oC) pre-treatment method on Starch casein agar media, from the soil sample that was collected nearer to the root region of the mangrove Avicennia marina from the back water area, Ariyankuppam, Puducherry (UT). It was identified as Streptomyces cacaoi subsp. cacaoi M20 by the 16S RNA sequencing technique. The isolate was keratinase and protease positive. The isolate utilized the small whole chicken feathers within 10-15 days. It was witnessed that the initial pH of the medium was 7.5, but the pH of the medium was raised from the day when the isolate started to utilize the keratin. Finally the pH of medium was measured as 8.8, more alkali, due to conversion of complex keratin into ammonia.