In the present study, the Ashwagandha plant as a natural source has been extensively utilized for antimicrobial activity. Withania somnifera (L.) is a nontoxic popular medicinal plant in Ayurvedic medicine also used for antifungal activity, antibacterial activity and antiviral activity. The Principle active compounds include several withanolide-type compounds. Various plant parts, like roots and fewer, often leaves and fruits of Ashwagandha, are used as plant-derived medicines. The potent antimicrobial activity against the phytopathogenic fungi and bacteria tested. The protein designated WSG (Withania somnifera glycoprotein) which has seen possesses the antimicrobial activity. The inhibition of virus at a maximum of 99.9 was shown by the hydroalcoholic extract of Withania somnifera roots, studied of its highest nontoxic concentration, 25μg/ml in Cytopathic Effect Reduction Assay. By compiling the results indicated the antiviral property Withania somnifera root extract against Infectious Bursal Disease virus.