Hathras district belong to the division of Aligarh, Uttar Pradesh, (India) is inhibited by a large number of rural population depends on the ethnomedicinal plants remarkably important medicinal source also. Present investigation relates to the plant based medicine used by the rural population and other communities of Hathras district for eliminating and controlling many diseases. Through investigation a total number of 15 plants species have been recorded which are used to cure various diseases. Some Indian Pharmaceutical companies, Vaidhyas, Dai and rural population are also use these plants for preparation of various Ayurvedic, Unani, Herbal, Homeopathy medicines etc.